Tricreatine: Uses of malate and laboratory ingredient identification

Most tricreatine malate is transported by albumin or immunoglobulin and dispersed into cells by concentration gradients. In cells, tricreatine malate is converted to FMN by flavin kinase, and FMN is converted to fad by fad synthase, which is the main form of tricreatine malate in tissues. Tricreatine malate exceeds the body's needs and is mainly excreted by the kidneys, and some metabolites are also excreted by the kidneys. The half-life of the drug is 66-84 minutes. The following will give you a detailed introduction to the use of tricreatine malate and the identification of laboratory components.

The main uses of tricreatine malate are as follows:

1. Promote cell development and regeneration, especially for people with certain trauma, adding tricreatine malate to the regular dose during the recuperation period can promote wound healing, and also help prevent wound inflammation.

2. It can also promote the continuous growth of hair, nails and skin;

3. It helps to prevent and eliminate the inflammatory response of the mouth, tongue and lips, also known as oral reproductive syndrome;

4. It helps to reduce eye fatigue and enhance vision, especially for people who use more eyes, you can take tricreatine malate regularly to avoid eye fatigue.

5. Affect the body's absorption of iron.

Tricreatine malate is available as a riboflavin supplement and can be used to treat oral keratitis, cheilitis, glossitis, conjunctivitis, and scrotitis caused by tricreatine malate deficiency.

Tricreatine malate laboratory test component identification results:

Through identification, it was found that the solubility of tricreatine malate was soluble in water, but insoluble in ethanol. According to the results of OT-42 assay. The samples were vacuum-dried with phosphorus pentoxide at 100°C for 5 h °C, and a 1.5% (w/v) sample solution was prepared with 20% (w/v) hydrochloric acid as the solvent, which was determined by conventional methods. It [α] D20 should be +38°~+42°. Determination of sodium in sulfate ash by the IT-28 method. The maximum absorbance ratio of the sample aqueous solution is 0.30-0.34 at 375 nm and 267 nm, and 0.35-0.40 at 444 nm and 26.7 nm.

Analysis of the content of internal components of creatine malate:

Tricreatine malate content analysis should be performed under non-direct light. Take 100mg of the sample and put it in a 500ml brown volumetric flask, add 100ml of water and 2.5ml of acetic acid, and use water to determine the volume. take 20.0ml of solution in a 200ml brown volumetric flask, add 3.5ml of 1.4% (w/v) sodium acetate solution, and use water to determine the volume. Determine the maximum absorbance of the solution at 444 nm (a). Total amount of pigment = (a× 5000/328 × W) × 1.367 where W is the sample amount, G. The toxicity of ADI is 0-0.5 mg/kg (FAO/WHO, 2001). LD50510mg/kg (intradermal injection in mice). GRAS (Food and Drug Administration § 182.56972000), use limit GB 1-4880-94: same as "01022, tricreatine malate". 2. FAO/WHO (1988): Synthetic products are limited to use as pigments. U.S. Food and Drug Administration § 184.1697 (2000): GMP Restrictions.